The high performance liquid chromatograph quoted the theory of the gas chromatogram, is made up of several parts such as accumulator, pump, injector, column, detector, recorder. The following is mainly to tell you about the characteristics of the high performance liquid chromatograph. Features 1. High pressure: Liquid chromatography uses liquid as the mobile phase (called carrier liquid). The liquid flows through the column and is subject to high resistance. In order to quickly pass through the column, high pressure must be applied to the carrier liquid. Generally up to 150 ~ 350 × 105Pa.
2. High speed: The flow rate of the mobile phase in the column is much faster than that of the conventional chromatography, generally up to 1 to 10 ml/min. The analytical time required for high performance liquid chromatography is much less than that of classical liquid chromatography, generally less than 1 h.
3. Efficient: Many new types of stationary phases have been researched recently, which greatly improves the separation efficiency.
4. High sensitivity: High-sensitivity detectors have been widely used in high performance liquid chromatography to further increase the sensitivity of the analysis. Such as fluorescence detector sensitivity up to 10-11g. In addition, the sample size is small, usually a few microliters.
5. Wide adaptability: Comparison between gas chromatography and high performance liquid chromatography: Although gas chromatography has advantages of good separation ability, high sensitivity, fast analysis speed, and easy operation, it is subject to technical conditions and the boiling point is too high. Substances or materials with poor thermal stability are difficult to analyze using gas chromatography. High performance liquid chromatography, on the other hand, only requires the sample to be made into a solution without the need for gasification and is therefore not limited by the volatility of the sample. For high boiling point, poor thermal stability, relatively high molecular weight (greater than 400) organic substances (these substances account for almost 75% to 80% of the total number of organic matter), in principle, high-performance liquid chromatography can be used for separation and analysis. According to statistics, about 20% of known compounds can be analyzed by gas chromatography, and about 70 to 80% can be analyzed by liquid chromatography.
High performance liquid chromatography can be divided into high performance gel chromatography, hydrophobic high performance liquid chromatography, reversed phase high performance liquid chromatography, high performance ion exchange liquid chromatography, high performance affinity liquid chromatography and high-efficiency focusing liquid phase according to the nature of its stationary phase. Chromatography and other types. The principle of separation or analysis of various compounds by different types of high performance liquid chromatography is basically similar to the principle of corresponding general liquid chromatography. The difference is that the HPLC is sensitive, fast, high resolution, and reproducible, and must be performed in the chromatograph.
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